10.1371/journal.pgen.1002682.g002 Yasuyuki Arakane Yasuyuki Arakane Joseph Lomakin Joseph Lomakin Stevin H. Gehrke Stevin H. Gehrke Yasuaki Hiromasa Yasuaki Hiromasa John M. Tomich John M. Tomich Subbaratnam Muthukrishnan Subbaratnam Muthukrishnan Richard W. Beeman Richard W. Beeman Karl J. Kramer Karl J. Kramer Michael R. Kanost Michael R. Kanost TcCPR27 and TcCPR18 are abundant in cuticle of the pronotum. Public Library of Science 2012 tccpr18 abundant cuticle 2012-04-26 02:02:49 Figure https://plos.figshare.com/articles/figure/_TcCPR27_and_TcCPR18_are_abundant_in_cuticle_of_the_pronotum_/317369 <p>To suppress cuticle tanning, dsRNA for <i>TcLac2</i> (2 ng per insect) was injected into day 0 pupae. Proteins from the pronotum and elytra were extracted from five adults at 0–30 min after eclosion, and the extracts were analyzed by SDS-PAGE (left panel). In the ds<i>Ver</i>-treated control animals, the extent of tanning of the pronotum was substantially greater than that of the elytron (right panel). S: PBS homogenate supernatant, P: PBS homogenate pellet.</p>