Seki, Shinsuke Mazur, Peter Protocol of each cooling procedure. *<p>1/4 ml sample straw was covered with a 1/2 ml straw, a 7 mm OD ×90 mm glass tube, and a 10 mm OD ×90 mm glass tube.</p>†<p>The Cryotop was covered with the Cryotop-cap-straw and a 7 mm OD ×90 mm glass tube.</p>‡<p>The straw or Cryotop were placed horizontally on a Styrofoam disk (14 cm diameter, 1.5 cm thick) floated on the surface of LN<sub>2</sub> in a 41 Dewar flask for >5 min before being immersed in LN<sub>2</sub>.</p>§<p>The cooling rates were determined from 20 to −120°C. N = 5.</p>¶<p>The cooling rates were determined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036058#pone.0036058-Seki1" target="_blank">[14]</a>.</p> cooling 2013-02-20
    https://plos.figshare.com/articles/dataset/_Protocol_of_each_cooling_procedure_/316271
10.1371/journal.pone.0036058.t003