%0 Generic %A Seki, Shinsuke %A Mazur, Peter %D 2013 %T Protocol of each cooling procedure. %U https://plos.figshare.com/articles/dataset/_Protocol_of_each_cooling_procedure_/316271 %R 10.1371/journal.pone.0036058.t003 %2 https://plos.figshare.com/ndownloader/files/645767 %K cooling %X *
1/4 ml sample straw was covered with a 1/2 ml straw, a 7 mm OD ×90 mm glass tube, and a 10 mm OD ×90 mm glass tube.
†The Cryotop was covered with the Cryotop-cap-straw and a 7 mm OD ×90 mm glass tube.
‡The straw or Cryotop were placed horizontally on a Styrofoam disk (14 cm diameter, 1.5 cm thick) floated on the surface of LN2 in a 41 Dewar flask for >5 min before being immersed in LN2.
§The cooling rates were determined from 20 to −120°C. N = 5.
¶The cooling rates were determined in [14].
%I PLOS ONE