%0 Figure %A Ren, Sheng %A Wang, Jun %A Chen, Tie-Long %A Li, Hao-Yu %A Wan, Yu-Shun %A Peng, Nan-Fang %A Gui, Xi-En %A Zhu, Ying %D 2016 %T NF-κB is involved in the inhibition of IFN-α by FN. %U https://plos.figshare.com/articles/figure/NF_B_is_involved_in_the_inhibition_of_IFN_by_FN_/3152968 %R 10.1371/journal.pone.0152721.g007 %2 https://plos.figshare.com/ndownloader/files/4911292 %K hepatitis B Virus Stimulated Fibronectin Facilitates Viral Maintenance %K Distinct Mechanisms Fibronectin %K hepatitis B virus %K weight extracellular matrix protein %K hepatic cell lines Huh 7 %K IFN %K protein kinase 1 %K HBV DNA replication %K transcriptional factor specificity protein 1 %K NF %K TAK 1 phosphorylation %K FN expression %K support HBV expression %K HepG 2. HBV infection %X

(A) NC-KD cells were transfected with pNF-κB-Luc (5 ×NF-κB binding site promoter driven luciferase reporter plasmid). At 24 h post transfection, cells were mock infected or SeV (MOI = 1) infected along with the treatment of indicated concentration of BAY-11 (μM). Cells were harvested for luciferase assay at 48 h post transfection. (B) NC-KD or FN-KD cells were mock infected or Sev (MOI = 1) infected. At 24 h post infection, cell culture were replaced with medium containing DMSO or BAY-11 (1 μM) for another 24 h. IFN-α mRNA level was determined by qRT-PCR. (C) NC-KD and FN-KD cells were transfected with pNF-κB-Luc. At 24 h post transfection, cells were mock infected or SeV (MOI = 1) infected. Cells were harvested for luciferase assay at 48 h post transfection.(D) NC-KD and FN-KD cells were mock infected or SeV (MOI = 1) infected for 12 h. Cytoplasmic and nuclear fractions were prepared for western blot with indicated antibodies to determine the localization of NF-κB subunits. All experiments were repeated at least three times with similar results. Data represent means ± SD, n = 3 (*p<0.05).

%I PLOS ONE