10.1371/journal.pone.0151839
Cherryl O. Talaue
Cherryl
O. Talaue
Ricardo C. H. del Rosario
Ricardo
C. H. del Rosario
Friedhelm Pfeiffer
Friedhelm
Pfeiffer
Eduardo R. Mendoza
Eduardo
R. Mendoza
Dieter Oesterhelt
Dieter
Oesterhelt
Model Construction and Analysis of Respiration in <i>Halobacterium salinarum</i>
Public Library of Science
2016
potassium uniport
ATP
proton motive force generation
electron transport chain
oxidative phosphorylation
archaeon Halobacterium salinarum
model parameters
2016-03-24 06:45:16
Dataset
https://plos.figshare.com/articles/dataset/Model_Construction_and_Analysis_of_Respiration_in_i_Halobacterium_salinarum_i_/3138337
<div><p>The archaeon <i>Halobacterium salinarum</i> can produce energy using three different processes, namely photosynthesis, oxidative phosphorylation and fermentation of arginine, and is thus a model organism in bioenergetics. Compared to its bacteriorhodopsin-driven photosynthesis, less attention has been devoted to modeling its respiratory pathway. We created a system of ordinary differential equations that models its oxidative phosphorylation. The model consists of the electron transport chain, the ATP synthase, the potassium uniport and the sodium-proton antiport. By fitting the model parameters to experimental data, we show that the model can explain data on proton motive force generation, ATP production, and the charge balancing of ions between the sodium-proton antiporter and the potassium uniport. We performed sensitivity analysis of the model parameters to determine how the model will respond to perturbations in parameter values. The model and the parameters we derived provide a resource that can be used for analytical studies of the bioenergetics of <i>H. salinarum</i>.</p></div>