10.1371/journal.pone.0036704.g003 David Judah David Judah Alena Rudkouskaya Alena Rudkouskaya Ryan Wilson Ryan Wilson David E. Carter David E. Carter Lina Dagnino Lina Dagnino Altered expression of genes encoding growth factors and developmental regulators in ILK-deficient epidermis. Public Library of Science 2012 genes encoding factors regulators ilk-deficient 2012-05-04 00:54:20 Figure https://plos.figshare.com/articles/figure/_Altered_expression_of_genes_encoding_growth_factors_and_developmental_regulators_in_ILK_deficient_epidermis_/313260 <p>(<b>A, B</b>) Expression levels of selected transcripts encoding proteins associated with growth factors or pigmentation (panel A), or key factors in developmental epidermal pathways (panel B) in ILK-deficient <i>K14Cre-Ilk<sup>f/f</sup></i> epidermis, determined by qPCR. The results are expressed as the mean+SEM (n = 5). The differences in expression in ILK-deficient epidermis for each of transcripts shown reached statistical significance (<i>P</i><0.001, Student's t test) relative to the corresponding transcript levels in ILK-expressing <i>K14Cre-Ilk<sup>f/+</sup></i> epidermis (set to 1). (<b>C</b>) Protein lysates prepared from the skin of 3 day-old <i>K14Cre-Ilk<sup>f/f</sup></i> or <i>K14Cre-Ilk<sup>f/+</sup></i> mice (two animals of each genotype) were resolved by denaturing gel electrophoresis, followed by immunoblot analysis using antibodies against LGR5, or γ-tubulin, used as loading control. Lysates prepared from wild type mouse brain were used as a positive control for LGR5 expression.</p>