10.1371/journal.pone.0146982
Liang Bai
Liang
Bai
Qianwei Li
Qianwei
Li
Lingmei Li
Lingmei
Li
Yan Lin
Yan
Lin
Sihai Zhao
Sihai
Zhao
Weirong Wang
Weirong
Wang
Rong Wang
Rong
Wang
Yongqin Li
Yongqin
Li
Jiangbei Yuan
Jiangbei
Yuan
Chengjian Wang
Chengjian
Wang
Zhongfu Wang
Zhongfu
Wang
Jianglin Fan
Jianglin
Fan
Enqi Liu
Enqi
Liu
Plasma High-Mannose and Complex/Hybrid N-Glycans Are Associated with Hypercholesterolemia in Humans and Rabbits
Public Library of Science
2016
electrospray ionization mass spectrometry
plasma glycan profile
plasma levels
hypercholesterolemic patients
plasma cholesterol levels
2016-03-21 05:36:56
Dataset
https://plos.figshare.com/articles/dataset/Plasma_High_Mannose_and_Complex_Hybrid_N_Glycans_Are_Associated_with_Hypercholesterolemia_in_Humans_and_Rabbits/3129424
<div><p>N-glycans play important roles in various pathophysiological processes and can be used as clinical diagnosis markers. However, plasma N-glycans change and their pathophysiological significance in the setting of hypercholesterolemia, a major risk factor for atherosclerosis, is unknown. Here, we collected plasma from both hypercholesterolemic patients and cholesterol-fed hypercholesterolemic rabbits, and determined the changes in the whole-plasma N-glycan profile by electrospray ionization mass spectrometry. We found that both the hypercholesterolemic patients and rabbits showed a dramatic change in their plasma glycan profile. Compared with healthy subjects, the hypercholesterolemic patients exhibited higher plasma levels of a cluster of high-mannose and complex/hybrid N-glycans (mainly including undecorated or sialylated glycans), whereas only a few fucosylated or fucosylated and sialylated N-glycans were increased. Additionally, cholesterol-fed hypercholesterolemic rabbits also displayed increased plasma levels of high-mannose in addition to high complex/hybrid N-glycan levels. The whole-plasma glycan profiles revealed that the plasma N-glycan levels were correlated with the plasma cholesterol levels, implying that N-glycans may be a target for treatment of hypercholesterolemia.</p></div>