Suppression of Nrf2 and NQO1 protein expression by cortisol in oxidative stress-induced H4IIE cells. Denise V. Kratschmar Diego Calabrese Jo Walsh Adam Lister Julia Birk Christian Appenzeller-Herzog Pierre Moulin Chris E. Goldring Alex Odermatt 10.1371/journal.pone.0036774.g005 https://plos.figshare.com/articles/figure/_Suppression_of_Nrf2_and_NQO1_protein_expression_by_cortisol_in_oxidative_stress_induced_H4IIE_cells_/310402 <p>H4IIE cells were treated for 24 h with vehicle (DMSO), cortisone, sulforaphane or cortisone and sulforaphane in the presence or absence of H<sub>2</sub>O<sub>2</sub>. Cells were lysed, and equal protein amounts were used for Western blot analysis. Samples were probed for Nrf2 and NQO1 using actin as a loading control. The <i>lower panel</i> shows a densitometric analysis of Nrf2 (<i>left</i>) and NQO1 (<i>right</i>) protein normalized against β-actin. A representative experiment is shown.</p> 2012-05-11 00:06:42 nrf2 nqo1 cortisol oxidative stress-induced h4iie