%0 Figure %A N. Dietsch, Gregory %A Lu, Hailing %A Yang, Yi %A Morishima, Chihiro %A Q. Chow, Laura %A Disis, Mary L. %A M. Hershberg, Robert %D 2016 %T Caspase-1 activation and IL-18 induction by VTX-2337 leads to NK cell activation. %U https://plos.figshare.com/articles/figure/Caspase_1_activation_and_IL_18_induction_by_VTX_2337_leads_to_NK_cell_activation_/3080116 %R 10.1371/journal.pone.0148764.g004 %2 https://plos.figshare.com/ndownloader/files/4787200 %K monocytic cells %K TLR 8 %K IFN %K ADCC %K patient NK cells %K NKG 2D %K NLRP 3 inflammasome %K Fc %K CD 107a NLRP 3 activation %K killer cell activation %K VTX %K USAN %K metastatic SCCHN show %K activates NK cells %K TLR 8 Agonist %K TLR 8 stimulation %K TNF %K IL %K NCT %X

(A-D) The effects of caspase-1 inhibition on IL-1β, IL-18, IFNγ, and TNFα production by PBMC. Duplicate PBMC cultures from four healthy donors (indicated as D1, D2, D3 and D4) were treated overnight with medium (Ø), VTX-2337 (1 μM), or VTX-2337 plus z-VAD-FMK (10 μg/ml). Levels of IL-1β, IL-18, IFNγ, and TNFα in culture supernatant were measured by ELISA. (E-F) The effects of IL-18 blockade on VTX-2337-induced IFNγ and CD107a expression in NK cells. Summary graphs show the percentage of IFNγ+ and CD107a+ NK cells from PBMC cultured with medium (Ø), VTX-2337 (0.5 μM), or VTX-2337 and anti-IL18 neutralizing antibody (20 μg/ml) for 9 donors where each symbol represents one individual donor. The horizontal bar represents group the mean for the treatment. Data shown are the summary of four independent experiments from the 9 donors, *, p<0.05 by Wilcoxon signed-rank test.

%I PLOS ONE