10.1371/journal.pone.0037823.g006 Yichao Zhu Yichao Zhu Yinhui Tian Yinhui Tian Jun Du Jun Du Zhenzhen Hu Zhenzhen Hu Ling Yang Ling Yang Jiaojing Liu Jiaojing Liu Luo Gu Luo Gu Daam1 participates in the rearrangement of stress fibers in MDA-MB-231 and MCF-7 cells. Public Library of Science 2012 participates rearrangement fibers mda-mb-231 mcf-7 2012-05-24 01:07:52 Figure https://plos.figshare.com/articles/figure/_Daam1_participates_in_the_rearrangement_of_stress_fibers_in_MDA_MB_231_and_MCF_7_cells_/304072 <p>(A) <i>N</i>-Daam1 and C3 transferase disrupted the formation of actin stress fibers in MDA-MB-231 cells. MDA-MB-231 cells were transiently transfected with <i>N</i>-Daam1 or pre-treated with 10 ng/µL C3 transferase for 1 h. MDA-MB-231 cells as well as transiently transfected Daam1 mutants were grown on cover slips, and then incubated in culture medium containing 500 ng/mL rWnt5a for 4 h. Subsequently, cells were fixed and F-actin organization was analyzed by phalloidin staining. (C) ΔDAD-Daam1 enhanced the formation/maintenance of actin stress fibers in MCF-7. MCF-7 cells were transiently transfected with ΔDAD-Daam1, and then incubated in culture medium in the absence of Wnt5a. Magnification, ×400. (B, D) The percentage of formation of actin stress fibers was determined in MDA-MB-231 cells (B) and MCF-7 cells (D), as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037823#s2" target="_blank">Materials and Methods</a>. The average is shown as mean ± s.d.</p>