2°NC cells show distal-oriented movement after dissolving the cluster.

Takamiya, Masanari; Stegmaier, Johannes; Kobitski, Andrei Yu; Schott, Benjamin; Weger, Benjamin D.; Margariti, Dimitra; Delgado, Angel R. Cereceda; Gourain, Victor; Scherr, Tim; Yang, Lixin; Sorge, Sebastian; Otte, Jens C.; Hartmann, Volker; van Wezel, Jos; Stotzka, Rainer; Reinhard, Thomas; Schlunck, Günther; Dickmeis, Thomas; Rastegar, Sepand; Mikut, Ralf; Nienhaus, Gerd Ulrich; Strähle, Uwe

doi:10.1371/journal.pgen.1008774.s016

pgen.1008774.s016.pdf (3.34 MB)

2°NC cells show distal-oriented movement after dissolving the cluster.

journal contribution

posted on 2020-06-17, 17:43 authored by Masanari Takamiya, Johannes Stegmaier, Andrei Yu Kobitski, Benjamin Schott, Benjamin D. Weger, Dimitra Margariti, Angel R. Cereceda Delgado, Victor Gourain, Tim Scherr, Lixin Yang, Sebastian Sorge, Jens C. Otte, Volker Hartmann, Jos van Wezel, Rainer Stotzka, Thomas Reinhard, Günther Schlunck, Thomas Dickmeis, Sepand Rastegar, Ralf Mikut, Gerd Ulrich Nienhaus, Uwe Strähle

(A, B) Movement angle histogram for NC cells destined for proximal (A, 1°NC cells; n = 44) and distal (B, 2°NC cells; n = 37) sides of the eye. Movement trajectories of an individual NC cell were projected on a horizontal plane and the angle relative to the anteroposterior axis was measured every 10 time points (every 3 min 24 s). Each bin is 10°. Black arrows show median direction (56.5° and 83.6° for proximal and distal selection, respectively). Wallraff test of angular distances between two groups showed significant difference, p-value < 2.2x10^-16. (C, D) Centred tracks starting from 22 hpf for proximal (C, 1°NC; n = 44) and distal (D, 2°NC; n = 37) destined NC cells. The starting position is set to 0 (bottom centre). (C) NC cells on the proximal side of the eye show moderate distal-oriented movement after 22 hpf. (D) In contrast NC cells destined for the distal side of the eye show higher displacement toward the distal end. (E-K) Cell morphology of 1°NC and 2°NC cells. (E-F) A 18-hpf embryo from Tg(sox10:mem-tdEosFP), highlighting NC cells in green. (E) 3D-overview of the optic cup region (stippled circle). (F) Projected focal planes for the proximal side of the eye (stippled region). Note the cuboidal or hexagonal cell shape of 1°NC cells retaining large areas of cell-cell contact with neighbouring cells. (G-K) Overview of a 26-hpf Tg(sox10:mem-tdEosFP) embryo (G, maximum projection) and magnified single cell morphology of NC cells in a single focal plane for proximal (H-I) and distal (J-K) ocular NC cells. Squares in G show individual locations of magnified cells in H-K. 1°NC cells appear flat and rectangular (arrows), while 2°NC cells show mesenchymal morphology with filopodia-like cellular protrusions (arrowheads). Orientation: dorsal (D), ventral (V), rostral (R) and caudal (C); Ln: lens; Scale bars: 20 μm.

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