10.1371/journal.pone.0038104.g001
Huma Safdar
Huma
Safdar
Ka Lei Cheung
Ka Lei
Cheung
Hans L. Vos
Hans
L. Vos
Frank J. Gonzalez
Frank J.
Gonzalez
Pieter H. Reitsma
Pieter
H. Reitsma
Yusuke Inoue
Yusuke
Inoue
Bart J. M. van Vlijmen
Bart
J. M. van Vlijmen
Screening and validation of siRNAs in mouse primary hepatocytes.
Public Library of Science
2013
validation
sirnas
2013-02-20 04:19:21
Figure
https://plos.figshare.com/articles/figure/_Screening_and_validation_of_siRNAs_in_mouse_primary_hepatocytes_/299166
<p>Screening of siRNA in mouse primary hepatocytes 24 hours after transfection with 0.3, 3 and 30nM of three <i>Hnf4a</i>-specific (#1–3, panel A), three <i>Cebpa</i> -specific siRNAs (#1–3, panel B) or a control siRNA (siNEG, panel A and B, open bars). <i>Hnf4a</i> and <i>Cebpa</i> transcript levels were determined by quantitative real-time PCR. β-actin was used as internal control for quantification and normalization. The ΔCt values of the individual samples were related to the mean ΔCt of the reference group (siNEG). On the x-axis siRNA concentrations are indicated. Data are expressed as mean with error bars representing the difference between 2 POWER of upper and lower range of the mean ΔΔCt (difference 2∧ΔΔCt+SEM and 2∧ΔΔCt−SEM). Individual experiments were performed in triplicate. Data were statistically analyzed using the Student's t-test. <i>P</i>-values <0.05 were regarded as statistically significant. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001.</p>