10.1371/journal.pntd.0001687.g001 Daniel R. Abánades Daniel R. Abánades Leonardo V. Arruda Leonardo V. Arruda Elaine S. Arruda Elaine S. Arruda José Roberto A. S. Pinto José Roberto A. S. Pinto Mario S. Palma Mario S. Palma Dorlene Aquino Dorlene Aquino Arlene J. Caldas Arlene J. Caldas Manuel Soto Manuel Soto Aldina Barral Aldina Barral Manoel Barral-Netto Manoel Barral-Netto IgG reaction pattern associated to DTH conversion. Public Library of Science 2012 dth 2012-06-19 00:19:06 Figure https://plos.figshare.com/articles/figure/_IgG_reaction_pattern_associated_to_DTH_conversion_/291146 <p>Total protein extract from <i>L. chagasi</i> was SDS-PAGE resolved (10% polyacrilamide) and electrotransferred to nitrocellulose filter. In all blots, sera were used at 1∶1000, and secondary anti-human IgG was used at 1∶2000. A) Sera from six VL patients before and after DTH+ conversion. B) Western blot results using sera from active VL and recovered VL patients, asymptomatic infected and non-infected individuals. Molecular weight markers are shown on the left panel.</p>