Dose-response effects of chemerin on the production of cytokines by activated peritoneal macrophages.
Benjamin Bondue
Olivier De Henau
Souphalone Luangsay
Thalie Devosse
Patricia de Nadaï
Jean-Yves Springael
Marc Parmentier
Olivier Vosters
10.1371/journal.pone.0040043.g003
https://plos.figshare.com/articles/figure/_Dose_response_effects_of_chemerin_on_the_production_of_cytokines_by_activated_peritoneal_macrophages_/286931
<p>Peritoneal macrophages from WT (white bars) or ChemR23<sup>−/−</sup> (black bars) mice, collected following i.p. Bio-Gel injection and selected by adherence, were tested for their production of pro-inflammatory (IL-6, IL-1β and TNF-α) and anti-inflammatory (IL-10) cytokines in response to stimulation by LPS and IFN-γ, in the presence or not of graded concentrations of recombinant chemerin (from 10<sup>−12</sup> to 10<sup>−6</sup> M). After 15 h of culture, supernatants were collected and cytokine levels were determined by ELISA. Results are expressed as the percentage of the LPS/IFN-γ-induced levels (left panels) or as pg/ml (right panels) and represent the mean ± SEM of 3 to 4 independent experiments.</p>
2012-06-29 01:55:31
chemerin
cytokines
activated
peritoneal