Bavamian, Sabine Pontes, Helena Cancela, José Charollais, Anne Startchik, Sergei Van De Ville, Dimitri Meda, Paolo Glibenclamide and quinine differentially modulate the intercellular synchronization of glucose-induced Ca<sup>2+</sup> oscillations. <p>(<i>A</i>) During stimulation by 20 mM glucose in the presence of 15 mM TEA, MIN6 cells exposed for 24 h to 10 µM glibenclamide showed increased proportion of synchronous cells (<i>gray bars</i>) compared to control, but decreased proportions of asynchronous (<i>white bars</i>) and silent cells (<i>black bars</i>); (<i>B</i>) Under the same conditions, MIN6 cells exposed for 24 h to 10 µM quinine, showed a reduced proportion of synchronous cells (<i>gray bars</i>). Bars show means + SE values of three independent experiments. *p<0.05 versus values of WT MIN6 cells; (<i>C</i>) The synchrony index of WT MIN6 cells, as evaluated using a NIPKOW confocal microscope, was about 0.6. This value was decreased in AS MIN6 cells, and increased after exposure of the cells to glibenclamide; (<i>D</i>) Similar values of synchrony index data were semi-automatically calculated, in the same cell types, using an ImageXpress microscope. Data are means + SE of seven clusters measured for each condition in C, and 87 clusters measured for each condition in D. *p<0.05, **p<0.01 and ***p<0.001 versus values of WT MIN6 cells.</p> quinine;differentially;modulate;intercellular;synchronization;glucose-induced 2012-07-25
    https://plos.figshare.com/articles/figure/_Glibenclamide_and_quinine_differentially_modulate_the_intercellular_synchronization_of_glucose_induced_Ca_2_oscillations_/273445
10.1371/journal.pone.0041535.g002