10.1371/journal.pone.0041535.g002 Sabine Bavamian Sabine Bavamian Helena Pontes Helena Pontes José Cancela José Cancela Anne Charollais Anne Charollais Sergei Startchik Sergei Startchik Dimitri Van De Ville Dimitri Van De Ville Paolo Meda Paolo Meda Glibenclamide and quinine differentially modulate the intercellular synchronization of glucose-induced Ca<sup>2+</sup> oscillations. Public Library of Science 2012 quinine differentially modulate intercellular synchronization glucose-induced 2012-07-25 00:57:25 Figure https://plos.figshare.com/articles/figure/_Glibenclamide_and_quinine_differentially_modulate_the_intercellular_synchronization_of_glucose_induced_Ca_2_oscillations_/273445 <p>(<i>A</i>) During stimulation by 20 mM glucose in the presence of 15 mM TEA, MIN6 cells exposed for 24 h to 10 µM glibenclamide showed increased proportion of synchronous cells (<i>gray bars</i>) compared to control, but decreased proportions of asynchronous (<i>white bars</i>) and silent cells (<i>black bars</i>); (<i>B</i>) Under the same conditions, MIN6 cells exposed for 24 h to 10 µM quinine, showed a reduced proportion of synchronous cells (<i>gray bars</i>). Bars show means + SE values of three independent experiments. *p<0.05 versus values of WT MIN6 cells; (<i>C</i>) The synchrony index of WT MIN6 cells, as evaluated using a NIPKOW confocal microscope, was about 0.6. This value was decreased in AS MIN6 cells, and increased after exposure of the cells to glibenclamide; (<i>D</i>) Similar values of synchrony index data were semi-automatically calculated, in the same cell types, using an ImageXpress microscope. Data are means + SE of seven clusters measured for each condition in C, and 87 clusters measured for each condition in D. *p<0.05, **p<0.01 and ***p<0.001 versus values of WT MIN6 cells.</p>