Engraftment of wild-type and <i>MyoD<sup>−/−</sup></i> myoblasts after myocardial injection into infarcted heart.
Yasuhiro Nakamura
Yoko Asakura
Bryan A. Piras
Hiroyuki Hirai
Christopher T. Tastad
Mayank Verma
Amanda J. Christ
Jianyi Zhang
Takanori Yamazaki
Minoru Yoshiyama
Atsushi Asakura
10.1371/journal.pone.0041736.g002
https://plos.figshare.com/articles/figure/_Engraftment_of_wild_type_and_MyoD_8722_8722_myoblasts_after_myocardial_injection_into_infarcted_heart_/273441
<p>(A) These panels show MI induced by left coronary artery ligation. Wild-type and <i>MyoD<sup>−/−</sup></i> myoblasts were directly injected into the peri-infarct regions of LV. After 1 week, X-gal staining of whole heart indicated that more <i>MyoD<sup>−/−</sup></i> myoblasts engrafted than wild-type myoblasts (arrows). Arrowheads indicate left coronary artery ligation points. X-gal staining of cross sections indicated that more <i>MyoD<sup>−/−</sup></i> myoblasts than wild-type myoblasts engrafted in both injured and uninjured areas of the heart. Arrows indicate engrafted lacZ<sup>+</sup> wild-type and <i>MyoD<sup>−/−</sup></i> myoblasts. Scale bars = 1 mm. (B) Engraftment of injected cells in the infarcted heart. More <i>MyoD<sup>−/−</sup></i> myoblasts than wild-type myoblasts engraft in the infarcted heart 3 day to 4 weeks post-transplantation. Y-axis indicates the survival rates of engrafted cells after an injection of 1×10<sup>6</sup> wild-type or <i>MyoD<sup>−/−</sup></i> myoblasts. (C) By day 3, anti-activated caspase-3 (Casp-3) and anti-phospho-histone H3 (p-His-H3) antibody staining demonstrated that lacZ<sup>+</sup> wild-type and <i>MyoD<sup>−/−</sup></i> myoblasts proliferate or undergo apoptotic cell death (n = 4). Nuclei were counter-stained with DAPI (blue). Scale bar = 50 µm. (D) Comparison of the relative numbers of lacZ<sup>+</sup>/activated caspase-3<sup>+</sup> or lacZ<sup>+</sup>/phospho-histone H3<sup>+</sup> cells for wild-type and <i>MyoD<sup>−/−</sup></i> myoblasts 3 days after transplantation.</p>
2013-02-20 01:53:30
wild-type
myoblasts
myocardial
injection
infarcted