Correlation between PTTG1 and miRNA-186-5p in invasion ability of HTR-8/SVneo cells. Seung Mook Lim Hee Yeon Jang Ji Eun Lee Joong Sik Shin Sun-Hwa Park Bo Hyun Yoon Gi Jin Kim 10.1371/journal.pone.0149371.g005 https://plos.figshare.com/articles/figure/Correlation_between_PTTG1_and_miRNA_186_5p_in_invasion_ability_of_HTR_8_SVneo_cells_/2732131 <p>(A) Expression of miRNA-186-5p in HTR-8/SVneo cells after 10 nM siPTTG1 treatment, analyzed by qRT-PCR. (B) Expression of miRNA-186-5p after miR-186-5p mimic or inhibitor treatment analyzed by qRT-PCR. (C) mRNA expression of PTTG1 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p. (D) Expression and density of PTTG1 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p. (E) Images and numbers of invaded HTR-8/SVneo cells exposed to 30 nM miR-186-5p (original magnification, ×100; scale bar = 80 μm). (F) Expression of MMP-9 and MMP-2 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p, analyzed by zymography. Values are means ± standard errors. *<i>p</i> < 0.05 and **<i>p</i> < 0.001. U6, β-actin, or GAPDH was used as a loading control.</p> 2016-02-22 09:24:07 placental development integrin beta 1 trophoblast invasion ability trophoblast invasion activity trophoblast invasion miRNA -186-5p PTTG 1 ITGB MMP expression ITGA PTTG 1 alteration Pituitary Tumor Transforming Gene polymerase chain reaction PTTG 1 expression MMP -9 expression Regulates Trophoblast Invasion