Correlation between PTTG1 and miRNA-186-5p in invasion ability of HTR-8/SVneo cells.
Seung Mook Lim
Hee Yeon Jang
Ji Eun Lee
Joong Sik Shin
Sun-Hwa Park
Bo Hyun Yoon
Gi Jin Kim
10.1371/journal.pone.0149371.g005
https://plos.figshare.com/articles/figure/Correlation_between_PTTG1_and_miRNA_186_5p_in_invasion_ability_of_HTR_8_SVneo_cells_/2732131
<p>(A) Expression of miRNA-186-5p in HTR-8/SVneo cells after 10 nM siPTTG1 treatment, analyzed by qRT-PCR. (B) Expression of miRNA-186-5p after miR-186-5p mimic or inhibitor treatment analyzed by qRT-PCR. (C) mRNA expression of PTTG1 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p. (D) Expression and density of PTTG1 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p. (E) Images and numbers of invaded HTR-8/SVneo cells exposed to 30 nM miR-186-5p (original magnification, ×100; scale bar = 80 μm). (F) Expression of MMP-9 and MMP-2 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p, analyzed by zymography. Values are means ± standard errors. *<i>p</i> < 0.05 and **<i>p</i> < 0.001. U6, β-actin, or GAPDH was used as a loading control.</p>
2016-02-22 09:24:07
placental development
integrin beta 1
trophoblast invasion ability
trophoblast invasion activity
trophoblast invasion
miRNA -186-5p
PTTG 1
ITGB
MMP expression
ITGA
PTTG 1 alteration
Pituitary Tumor Transforming Gene
polymerase chain reaction
PTTG 1 expression
MMP -9 expression
Regulates Trophoblast Invasion