Invasion abilities of HTR-8/SVneo cells exposed to siPTTG1.
Seung Mook Lim
Hee Yeon Jang
Ji Eun Lee
Joong Sik Shin
Sun-Hwa Park
Bo Hyun Yoon
Gi Jin Kim
10.1371/journal.pone.0149371.g002
https://plos.figshare.com/articles/figure/Invasion_abilities_of_HTR_8_SVneo_cells_exposed_to_siPTTG1_/2732101
<p>(A) Images and numbers of invaded HTR-8/SVneo cells after siPTTG1 treatment (original magnification, ×100; scale bar = 80 μm). (B) Expression and densities of MMP-9 and MMP-2 in HTR-8/SVneo cells analyzed by zymography. (C) Expression of MMP-9 and MMP-2 mRNA in HTR-8/SVneo cells analyzed by qRT-PCR. (D) Expression and densities of MMP-9 and MMP-2 in HTR-8/SVneo cells after siPTTG1 treatment analyzed by western blot. Values are means ± standard errors. *<i>p</i> < 0.05 and **<i>p</i> < 0.001. β-actin or GAPDH was used as a loading control.</p>
2016-02-22 09:24:07
placental development
integrin beta 1
trophoblast invasion ability
trophoblast invasion activity
trophoblast invasion
miRNA -186-5p
PTTG 1
ITGB
MMP expression
ITGA
PTTG 1 alteration
Pituitary Tumor Transforming Gene
polymerase chain reaction
PTTG 1 expression
MMP -9 expression
Regulates Trophoblast Invasion