Invasion abilities of HTR-8/SVneo cells exposed to siPTTG1. Seung Mook Lim Hee Yeon Jang Ji Eun Lee Joong Sik Shin Sun-Hwa Park Bo Hyun Yoon Gi Jin Kim 10.1371/journal.pone.0149371.g002 https://plos.figshare.com/articles/figure/Invasion_abilities_of_HTR_8_SVneo_cells_exposed_to_siPTTG1_/2732101 <p>(A) Images and numbers of invaded HTR-8/SVneo cells after siPTTG1 treatment (original magnification, ×100; scale bar = 80 μm). (B) Expression and densities of MMP-9 and MMP-2 in HTR-8/SVneo cells analyzed by zymography. (C) Expression of MMP-9 and MMP-2 mRNA in HTR-8/SVneo cells analyzed by qRT-PCR. (D) Expression and densities of MMP-9 and MMP-2 in HTR-8/SVneo cells after siPTTG1 treatment analyzed by western blot. Values are means ± standard errors. *<i>p</i> < 0.05 and **<i>p</i> < 0.001. β-actin or GAPDH was used as a loading control.</p> 2016-02-22 09:24:07 placental development integrin beta 1 trophoblast invasion ability trophoblast invasion activity trophoblast invasion miRNA -186-5p PTTG 1 ITGB MMP expression ITGA PTTG 1 alteration Pituitary Tumor Transforming Gene polymerase chain reaction PTTG 1 expression MMP -9 expression Regulates Trophoblast Invasion