ComA can process and secrete BlpC independently of BlpA. Wei-Yun Wholey Travis J. Kochan David N. Storck Suzanne Dawid 10.1371/journal.ppat.1005413.g005 https://plos.figshare.com/articles/figure/ComA_can_process_and_secrete_BlpC_independently_of_BlpA_/2609398 <p>(A-C) <i>BIR</i><sub><i>P1</i></sub><i>-lacZ</i> transcriptional response in wildtype (blue circles), <i>blpA</i><sub><i>FS</i></sub> (purple octagon) and <i>blpA</i><sub><i>FS</i></sub>/<i>comAB</i> (red squares) mutant reporters during growth in broth. Closed symbols denote CSP addition, open symbols denote no peptide added. Cellular growth is shown as grey lines. (A) Response to CSP added at time 0 in THY. (B-C) Natural <i>BIR</i><sub><i>P1</i></sub><i>-lacZ</i> induction in competence permissive (B) and competence non-permissive C+Y media (C). (D) Western blot of cell lysate of BlpC<sub>FLAG</sub> expressing strains in <i>ΔhtrA</i> background: PSD127 (Wt), PSD131 (<i>ΔblpA</i>), PSD146 (<i>ΔcomAB</i>) and PSD147 (<i>ΔblpA/comAB</i>) after treatment with CSP or BlpC<sub>R6</sub> or both BlpC<sub>R6</sub>/CSP. Preprocessed BlpC<sub>FLAG</sub> is denoted as pre-BlpC, the processed form is BlpC. Anti-pneumolysin (Ply) antibody was used as a loading control. Densitometry analysis was performed to determine % processed BlpC<sub>FLAG</sub> of the total BlpC<sub>FLAG</sub> detected in each strain.</p> 2016-02-03 23:00:38 Neighbor Predation Streptococcus pneumoniae extracellular accumulation operon encoding BlpC DNA exchange transporter mediates blp locus adaptive advantage mucosal surface Genetic Adaptation bacteriocin pheromone adaptation array Streptococcus pneumoniae Contributes mucosal disease incorporation DNA uptake bacteriocin secretion blp bacteriocins Bacteriocin Expression competence CSP stimulation results organism pneumococcu