%0 Figure %A Zhang, Kaihui %A Liu, Shu %A Feng, Bing %A Yang, Yali %A Zhang, Haiyan %A Dong, Rui %A Liu, Yi %A Gai, Zhongtao %D 2016 %T Schematic map of the MS-PCR and MS-PCR analysis for AS/PWS patients. %U https://plos.figshare.com/articles/figure/Schematic_map_of_the_MS_PCR_and_MS_PCR_analysis_for_AS_PWS_patients_/2605987 %R 10.1371/journal.pone.0147824.g003 %2 https://plos.figshare.com/ndownloader/files/4255393 %K Syndrome %K PCR %K syndrome %K 26 PWS patients %K 5 AS patients %K uniparental disomy %K deletion %K UPD %K STR linkage analysis %K chr 15q %K linkage analysis %K Angelman %K diagnosis %K genomic hybridization techniques %K disorder %X

A: Physical map of the SNRPN, UBE3A, and the snoRNA genes. Also shown is the general distribution of the sequence corresponding to the MS-PCR primers for PWS/AS detection. B: MS-PCR analysis of SNRPN gene of AS/PWS patients. MS-PCR products of 174 bp and 100 bp are amplified from methylated and unmethylated alleles of the SNRPN gene locus respectively. Case 1: Lane 1, 100-bp DNA ladder marker; Lane 2, patien’s father; Lane 3, patient’s mother; Lane 4 and 5, case 1 (AS), Lane 6, negative control; Lane 7, AS positive control; Lane 8, PWS positive control; Lane 9, blank control; Lane 10, untreated genomic DNA control. Case 6: Lane 1, 100-bp DNA ladder marker; Lane 2, patient's father; Lane 3, patient's mother; Lane 4 and 5, case 6, PWS, UPD type; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control; Lane 10, untreated genomic DNA control. Case 31: Lane 1, 100-bp DNA ladder marker; Lane 2, patient's father; Lane 3, patient's mother; Lane 4 and 5, case 31, PWS, deletion type; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control; Lane 10, untreated genomic DNA control.

%I PLOS ONE