%0 Figure %A K. Johnson, Eric %A Zhang, Liwen %A E. Adams, Marvin %A Phillips, Alistair %A Freitas, Michael A. %A C. Froehner, Stanley %A Green-Church, Kari B. %A Montanaro, Federica %D 2012 %T Differences in α-dystrobrevin splice variants between cardiac and skeletal muscle DAPC. %U https://plos.figshare.com/articles/figure/_Differences_in_945_dystrobrevin_splice_variants_between_cardiac_and_skeletal_muscle_DAPC_/258040 %R 10.1371/journal.pone.0043515.g004 %2 https://plos.figshare.com/ndownloader/files/587554 %K splice %K variants %K cardiac %K skeletal %X

(A) Western blot analysis of α-dystrobrevins in mouse cardiac (C) and skeletal (S) muscle total protein lysates, DYS-IPs and IgG-IPs. α3-dystrobrevin associates with dystrophin in the heart. Fold differences in α-dystrobrevin abundance in cardiac vs. skeletal muscle DYS-IPs relative to dystrophin are shown (averages ±SD, N = 3). (B) Immunolabeling of wild type cardiac sections for α1 and α2-dystrobrevins. Scale bar: 50 µm. (C) Immunolabeling of mdx cardiac tissue section for β-sarcoglycan. Scale bar: 50 µm. (D) Western blot analysis of α-dystrobrevins in heart protein lysates from wild type (WT) and mdx mice. Fold differences in α-dystrobrevin abundance in WT vs. mdx cardiac lysates relative to GAPDH are shown (averages ±SD, N = 3) (E) Western blot analysis of α-dystrobrevins in DYS-IPs from human (H) and mouse (M) cardiac samples. Additional α-dystrobrevin isoforms (arrow heads) are detected in human cardiac lysates and DYS-IP.

%I PLOS ONE