10.1371/journal.pone.0043515.g004 Eric K. Johnson Eric K. Johnson Liwen Zhang Liwen Zhang Marvin E. Adams Marvin E. Adams Alistair Phillips Alistair Phillips Michael A. Freitas Michael A. Freitas Stanley C. Froehner Stanley C. Froehner Kari B. Green-Church Kari B. Green-Church Federica Montanaro Federica Montanaro Differences in α-dystrobrevin splice variants between cardiac and skeletal muscle DAPC. Public Library of Science 2012 splice variants cardiac skeletal 2012-08-24 02:14:00 Figure https://plos.figshare.com/articles/figure/_Differences_in_945_dystrobrevin_splice_variants_between_cardiac_and_skeletal_muscle_DAPC_/258040 <p>(<b>A</b>) Western blot analysis of α-dystrobrevins in mouse cardiac (C) and skeletal (S) muscle total protein lysates, DYS-IPs and IgG-IPs. α3-dystrobrevin associates with dystrophin in the heart. Fold differences in α-dystrobrevin abundance in cardiac vs. skeletal muscle DYS-IPs relative to dystrophin are shown (averages ±SD, N = 3). (<b>B</b>) Immunolabeling of wild type cardiac sections for α1 and α2-dystrobrevins. Scale bar: 50 µm. (<b>C</b>) Immunolabeling of <i>mdx</i> cardiac tissue section for β-sarcoglycan. Scale bar: 50 µm. (<b>D</b>) Western blot analysis of α-dystrobrevins in heart protein lysates from wild type (WT) and <i>mdx</i> mice. Fold differences in α-dystrobrevin abundance in WT vs. mdx cardiac lysates relative to GAPDH are shown (averages ±SD, N = 3) (<b>E</b>) Western blot analysis of α-dystrobrevins in DYS-IPs from human (H) and mouse (M) cardiac samples. Additional α-dystrobrevin isoforms (arrow heads) are detected in human cardiac lysates and DYS-IP.</p>