10.1371/journal.pone.0043515.g004
Eric K. Johnson
Eric
K. Johnson
Liwen Zhang
Liwen
Zhang
Marvin E. Adams
Marvin
E. Adams
Alistair Phillips
Alistair
Phillips
Michael A. Freitas
Michael A.
Freitas
Stanley C. Froehner
Stanley
C. Froehner
Kari B. Green-Church
Kari B.
Green-Church
Federica Montanaro
Federica
Montanaro
Differences in α-dystrobrevin splice variants between cardiac and skeletal muscle DAPC.
Public Library of Science
2012
splice
variants
cardiac
skeletal
2012-08-24 02:14:00
Figure
https://plos.figshare.com/articles/figure/_Differences_in_945_dystrobrevin_splice_variants_between_cardiac_and_skeletal_muscle_DAPC_/258040
<p>(<b>A</b>) Western blot analysis of α-dystrobrevins in mouse cardiac (C) and skeletal (S) muscle total protein lysates, DYS-IPs and IgG-IPs. α3-dystrobrevin associates with dystrophin in the heart. Fold differences in α-dystrobrevin abundance in cardiac vs. skeletal muscle DYS-IPs relative to dystrophin are shown (averages ±SD, N = 3). (<b>B</b>) Immunolabeling of wild type cardiac sections for α1 and α2-dystrobrevins. Scale bar: 50 µm. (<b>C</b>) Immunolabeling of <i>mdx</i> cardiac tissue section for β-sarcoglycan. Scale bar: 50 µm. (<b>D</b>) Western blot analysis of α-dystrobrevins in heart protein lysates from wild type (WT) and <i>mdx</i> mice. Fold differences in α-dystrobrevin abundance in WT vs. mdx cardiac lysates relative to GAPDH are shown (averages ±SD, N = 3) (<b>E</b>) Western blot analysis of α-dystrobrevins in DYS-IPs from human (H) and mouse (M) cardiac samples. Additional α-dystrobrevin isoforms (arrow heads) are detected in human cardiac lysates and DYS-IP.</p>