10.1371/journal.pone.0147549
Qiang Huang
Qiang
Huang
Yan Ping Chen
Yan
Ping Chen
Rui Wu Wang
Rui
Wu Wang
Shang Cheng
Shang
Cheng
Jay D. Evans
Jay
D. Evans
Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees
Public Library of Science
2016
SNP positions
ceranae strains
infection period
Nosema ceranae parasitism
reactive mitochondrial oxygen species modulator 1
parasite genome
Honey Bees
time period
parasite mRNAs
honey bee cells
1122 parasite genes
data support
reproduction cycle
4 expression patterns
honey bee host
Microsporidian Parasite
hypothesis
Purifying Selection
2016-02-03 12:31:36
Dataset
https://plos.figshare.com/articles/dataset/Host_Parasite_Interactions_and_Purifying_Selection_in_a_Microsporidian_Parasite_of_Honey_Bees/2577763
<div><p>To clarify the mechanisms of <i>Nosema ceranae</i> parasitism, we deep-sequenced both honey bee host and parasite mRNAs throughout a complete 6-day infection cycle. By time-series analysis, 1122 parasite genes were significantly differently expressed during the reproduction cycle, clustering into 4 expression patterns. We found reactive mitochondrial oxygen species modulator 1 of the host to be significantly down regulated during the entire infection period. Our data support the hypothesis that apoptosis of honey bee cells was suppressed during infection. We further analyzed genome-wide genetic diversity of this parasite by comparing samples collected from the same site in 2007 and 2013. The number of SNP positions per gene and the proportion of non-synonymous substitutions per gene were significantly reduced over this time period, suggesting purifying selection on the parasite genome and supporting the hypothesis that a subset of <i>N</i>. <i>ceranae</i> strains might be dominating infection.</p></div>