Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region Kiwan Kim Najung Kim Gap Ryol Lee 10.1371/journal.pone.0148576 https://plos.figshare.com/articles/dataset/Transcription_Factors_Oct_1_and_GATA_3_Cooperatively_Regulate_Th2_Cytokine_Gene_Expression_via_the_RHS5_within_the_Th2_Locus_Control_Region/2576860 <div><p>The T helper type 2 (Th2) locus control region (LCR) regulates Th2 cell differentiation. Several transcription factors bind to the LCR to modulate the expression of Th2 cytokine genes, but the molecular mechanisms behind Th2 cytokine gene regulation are incompletely understood. Here, we used database analysis and an oligonucleotide competition/electrophoretic mobility shift assays to search for transcription factors binding to RHS5, a DNase I hypersensitive site (DHS) within the Th2 LCR. Consequently, we demonstrated that GATA-binding protein-3 (GATA-3), E26 transformation-specific protein 1 (Ets-1), octamer transcription factor-1 (Oct-1), and Oct-2 selectively associate with RHS5. Furthermore, chromatin immunoprecipitation and luciferase reporter assays showed that Oct-1 and Oct-2 bound within the <i>Il4</i> promoter region and the Th2 LCR, and that Oct-1 and GATA-3 or Oct-2 synergistically triggered the transactivational activity of the <i>Il4</i> promoter through RHS5. These results suggest that Oct-1 and GATA-3/Oct-2 direct Th2 cytokine gene expression in a cooperative manner.</p></div> 2016-02-03 12:31:32 Th 2 cell differentiation Several transcription factors bind Transcription Factors Oct Th 2 cytokine gene regulation T helper type 2 transcription factors binding GATA Th 2 cytokine gene expression 4 promoter region Th 2 Locus Control Region RHS 5. luciferase reporter assays Th 2 LCR Th 2 cytokine genes DHS