10.1371/journal.pone.0041592.g004
Evandro Fei Fang
Evandro
Fei Fang
Chris Zhi Yi Zhang
Chris
Zhi Yi Zhang
Lin Zhang
Lin
Zhang
Jack Ho Wong
Jack Ho
Wong
Yau Sang Chan
Yau
Sang Chan
Wen Liang Pan
Wen
Liang Pan
Xiu Li Dan
Xiu
Li Dan
Cui Ming Yin
Cui
Ming Yin
Chi Hin Cho
Chi Hin
Cho
Tzi Bun Ng
Tzi Bun
Ng
The activation of caspase cascades was involved in TCS-induced cell apoptosis.
Public Library of Science
2013
activation
caspase
cascades
was
tcs-induced
2013-02-19 23:47:53
Figure
https://plos.figshare.com/articles/figure/_The_activation_of_caspase_cascades_was_involved_in_TCS_induced_cell_apoptosis_/252129
<p>A. After treatment with TCS at the indicated concentrations, cells were harvested and protein levels of caspase-8, caspase-9, caspase-3, and PARP were determined by western blotting. Right panel, quantitative data from two independent experiments. Lower panel, The caspase-8 and caspase-9 activities were determined by ELISA kits. Asterisk, <i>p</i><0.05 compared with control. B. TCS increased the number of tumor cells undergoing mitochondrial membrane depolarization. Cells were treated with TCS at the indicated concentrations for 24 h, and flow cytometry analysis of the mitochondrial membrane depolarization was carried out after JC-1 staining. C. Z-VAD-FMK pretreatment retarded TCS-induced cell apoptosis. After different treatments as indicated at the bottom of the histogram, cells were harvested, stained with Annexin V-PI, and processed for flow cytometry. Asterisk, <i>p</i><0.05 compared with control.</p>