10.1371/journal.pone.0044598.g003
María-Salud García-Ayllón
María-Salud
García-Ayllón
Cristina Millán
Cristina
Millán
Carol Serra-Basante
Carol
Serra-Basante
Ramón Bataller
Ramón
Bataller
Javier Sáez-Valero
Javier
Sáez-Valero
Increased levels of acetycholinesterase (AChE) mRNA variants in cirrhotic liver.
Public Library of Science
2012
levels
acetycholinesterase
mrna
variants
cirrhotic
2012-09-13 02:09:11
Figure
https://plos.figshare.com/articles/figure/_Increased_levels_of_acetycholinesterase_AChE_mRNA_variants_in_cirrhotic_liver_/247751
<p>Relative mRNA levels of the liver transcripts for AChE (T, R and H; labelled AChE-T, AChE-R and AChE-H in the figure) were analyzed by QRT-PCR in non-diseased (NL; ▪; n = 5) and cirrhotic liver cases (CL; □; n = 9). The selected primers were: AChE-T transcript: Fw: 5′-CTTCCTCCCCAAATTGCTC3-′, Rev: 5′-TCCTGCTTGCTGTAGTGGTC-3′; AChE-R transcript: Fw: 5′-CTTCCTCCCCAAATTGCTC-3′, Rev: 5′-GGGGAGAAGAGAGGGGTTAC-3′; AChE-H transcript: Fw: 5′-CAATGAGCCCCGAGACC-3′, Rev: 5′-GAGCCTCCGAGGCGGTG-3′; GAPDH: Fw: 5′-AGCCACATCGCTCAGACAC-3′, Rev: 5′-GCCCAATACGACCAAATCC-3′. Values were calculated using relative standard curves and normalized to GAPDH. Specificity of the PCR products was confirmed by dissociation curve analysis. *<i>p</i><0.05.</p>