10.1371/journal.pone.0044598.g003 María-Salud García-Ayllón María-Salud García-Ayllón Cristina Millán Cristina Millán Carol Serra-Basante Carol Serra-Basante Ramón Bataller Ramón Bataller Javier Sáez-Valero Javier Sáez-Valero Increased levels of acetycholinesterase (AChE) mRNA variants in cirrhotic liver. Public Library of Science 2012 levels acetycholinesterase mrna variants cirrhotic 2012-09-13 02:09:11 Figure https://plos.figshare.com/articles/figure/_Increased_levels_of_acetycholinesterase_AChE_mRNA_variants_in_cirrhotic_liver_/247751 <p>Relative mRNA levels of the liver transcripts for AChE (T, R and H; labelled AChE-T, AChE-R and AChE-H in the figure) were analyzed by QRT-PCR in non-diseased (NL; ▪; n = 5) and cirrhotic liver cases (CL; □; n = 9). The selected primers were: AChE-T transcript: Fw: 5′-CTTCCTCCCCAAATTGCTC3-′, Rev: 5′-TCCTGCTTGCTGTAGTGGTC-3′; AChE-R transcript: Fw: 5′-CTTCCTCCCCAAATTGCTC-3′, Rev: 5′-GGGGAGAAGAGAGGGGTTAC-3′; AChE-H transcript: Fw: 5′-CAATGAGCCCCGAGACC-3′, Rev: 5′-GAGCCTCCGAGGCGGTG-3′; GAPDH: Fw: 5′-AGCCACATCGCTCAGACAC-3′, Rev: 5′-GCCCAATACGACCAAATCC-3′. Values were calculated using relative standard curves and normalized to GAPDH. Specificity of the PCR products was confirmed by dissociation curve analysis. *<i>p</i><0.05.</p>