C. Sutherland, Molly Linh Nguyen, Thuy Tseng, Victor P. Vogel, Joseph Recruitment of IcmSW to the membrane by the <i>dotL</i> mutants. <p>Subcellular localization of IcmS was assayed using <i>L. pneumophila</i> lysates separated into total (T), soluble (S) and membrane (M) fractions. Δ<i>A</i> Δ<i>L</i> is the Δ<i>dotA</i> Δ<i>dotL</i> double mutant and all of the <i>dotL</i> mutants were expressed in a Δ<i>dotL</i> background. Protein fractions were analyzed by western blot using an IcmS-specific antibody. Fractions were loaded proportionally and are representative of three independent experiments.</p> icmsw;membrane 2012-09-13
    https://plos.figshare.com/articles/figure/_Recruitment_of_IcmSW_to_the_membrane_by_the_dotL_mutants_/247744
10.1371/journal.ppat.1002910.g002