10.1371/journal.pone.0045514.g007 Antje Banning Antje Banning Wymke Ockenga Wymke Ockenga Fabian Finger Fabian Finger Philipp Siebrasse Philipp Siebrasse Ritva Tikkanen Ritva Tikkanen Induction of endogenous flotillin expression by RAR, RXR, retinoic acid, and PPARγ. Public Library of Science 2013 endogenous flotillin retinoic 2013-02-19 23:16:16 Figure https://plos.figshare.com/articles/figure/_Induction_of_endogenous_flotillin_expression_by_RAR_RXR_retinoic_acid_and_PPAR_947_/246358 <p>Hela cells were transiently transfected with expression constructs for RAR, RXR, or a combination of both. Empty PSV vector served as a control. One day post-transfection, the cells were stimulated with trans-RA (1 µM) in serum-free medium for 24 h. Cell lysates were analyzed for flotillin-1 (A), flotillin-2 (B), RAR and RXR (C) by Western blotting. D and E show a densitometric quantification of flotillin expression. F: Cells were transfected with RAR or PPARγ expression construct or empty PSV. RNA was isolated, transcribed into cDNA and flotillin mRNA was measured by qPCR. Values are mean ± standard deviation of at least 3 experiments. ###, p<0.001; #, p<0.05; vs control *, p<0.05 vs. unstimulated sample.</p>