10.1371/journal.pone.0045514.g007
Antje Banning
Antje
Banning
Wymke Ockenga
Wymke
Ockenga
Fabian Finger
Fabian
Finger
Philipp Siebrasse
Philipp
Siebrasse
Ritva Tikkanen
Ritva
Tikkanen
Induction of endogenous flotillin expression by RAR, RXR, retinoic acid, and PPARγ.
Public Library of Science
2013
endogenous
flotillin
retinoic
2013-02-19 23:16:16
Figure
https://plos.figshare.com/articles/figure/_Induction_of_endogenous_flotillin_expression_by_RAR_RXR_retinoic_acid_and_PPAR_947_/246358
<p>Hela cells were transiently transfected with expression constructs for RAR, RXR, or a combination of both. Empty PSV vector served as a control. One day post-transfection, the cells were stimulated with trans-RA (1 µM) in serum-free medium for 24 h. Cell lysates were analyzed for flotillin-1 (A), flotillin-2 (B), RAR and RXR (C) by Western blotting. D and E show a densitometric quantification of flotillin expression. F: Cells were transfected with RAR or PPARγ expression construct or empty PSV. RNA was isolated, transcribed into cDNA and flotillin mRNA was measured by qPCR. Values are mean ± standard deviation of at least 3 experiments. ###, p<0.001; #, p<0.05; vs control *, p<0.05 vs. unstimulated sample.</p>