BRSK2 protein levels fluctuate during the cell cycle. Ruwei Li Bo Wan Jun Zhou Yingli Wang Ting Luo Xiuting Gu Fang Chen Long Yu 10.1371/journal.pone.0045932.g002 https://plos.figshare.com/articles/figure/_BRSK2_protein_levels_fluctuate_during_the_cell_cycle_/243508 <p>(A) HeLa cells were arrested at the G1-S boundary by a double-thymidine block, released into fresh medium, and harvested at the indicated time points. Cell lysates were probed by Western blot (WB) with BRSK2, Aurora B, Cyclin B1, and β-actin antibodies. β-actin served as a loading control. (B) HeLa cells were arrested at prometaphase by a thymidine-nocodazole treatment, released into fresh medium, and harvested at the indicated time points. Cell lysates were probed by Western blot (WB) with BRSK2, Aurora B, p-Histone H3(Ser10), and β-actin antibodies. Cells were treated with MG132 (20 µM) for 8 h during the mitotic exit phase. Cell cycle stages in (A) and (B) were determined by flow cytometry and are indicated in the figure.</p> 2013-02-19 23:00:42 levels fluctuate