Tcf-4 knockdown is more effective at inhibiting cell proliferation and inducing apoptosis than β-catenin knockdown in colon cancer cells. XieJiang XiangDe-Bing WangHong ZhaoCong ChenJie XiongFeng LiTing-Yu WangXiao-Lei 2013 <p>Cells were treated with adenoviruses (50 MOI) carrying shRNA for 24 h. <b>a,</b> Cell proliferation was determined using a colony formation assay. <b>b,</b> Cell apoptosis was determined using Annexin-V-FITC staining. <b>c</b>, The expression of proapoptotic enzyme caspase-3 was determined by western blot. Each data point represents the mean ± s.d. of three or more independent determinations. *<i>P</i><0.01 versus control shRNA; <sup>#</sup><i>P</i><0.05 versus β-catenin shRNA; <sup>##</sup><i>P</i><0.01 versus β-catenin shRNA.</p>