10.1371/journal.pone.0149208.g001 Xuguo Duan Xuguo Duan Sheng Cheng Sheng Cheng Yixin Ai Yixin Ai Jing Wu Jing Wu SDS-PAGE analysis of recombinant <i>S</i>. <i>plymuthica</i> AS9 PalI and its variants expressed in <i>E</i>. <i>coli</i> BL21(DE3). Public Library of Science 2016 BL enzyme Serratia plymuthica Sucrose Isomerase E 175N mutants AS 9 PalI Serratia plymuthica AS 9 577K E 175N E 175N K 576D K 174D G 176D S 575D E 175N K 576D E 175N K 576D E 175N K 576D mutants 2016-02-17 11:47:52 Figure https://plos.figshare.com/articles/figure/SDS_PAGE_analysis_of_recombinant_i_S_i_i_plymuthica_i_AS9_PalI_and_its_variants_expressed_in_i_E_i_i_coli_i_BL21_DE3_/2356321 <p>(A) <i>Lane</i> M, molecular weight markers; <i>Lane</i> 1, culture supernatant of the wild-type enzyme; Sucrose isomerase is indicated by the black arrow. (B) <i>Lane</i> M, molecular weight markers; <i>Lane</i> 1, purified wild-type enzyme; <i>Lane</i> 2, purified E175N; <i>Lane</i> 3, purified K576D; <i>Lane</i> 3, purified E175N/K576D.</p>