10.1371/journal.pone.0149208.g001
Xuguo Duan
Xuguo
Duan
Sheng Cheng
Sheng
Cheng
Yixin Ai
Yixin
Ai
Jing Wu
Jing
Wu
SDS-PAGE analysis of recombinant <i>S</i>. <i>plymuthica</i> AS9 PalI and its variants expressed in <i>E</i>. <i>coli</i> BL21(DE3).
Public Library of Science
2016
BL
enzyme
Serratia plymuthica Sucrose Isomerase
E 175N mutants
AS 9 PalI
Serratia plymuthica AS 9
577K
E 175N
E 175N K 576D K 174D G 176D S 575D
E 175N K 576D
E 175N K 576D E 175N
K 576D mutants
2016-02-17 11:47:52
Figure
https://plos.figshare.com/articles/figure/SDS_PAGE_analysis_of_recombinant_i_S_i_i_plymuthica_i_AS9_PalI_and_its_variants_expressed_in_i_E_i_i_coli_i_BL21_DE3_/2356321
<p>(A) <i>Lane</i> M, molecular weight markers; <i>Lane</i> 1, culture supernatant of the wild-type enzyme; Sucrose isomerase is indicated by the black arrow. (B) <i>Lane</i> M, molecular weight markers; <i>Lane</i> 1, purified wild-type enzyme; <i>Lane</i> 2, purified E175N; <i>Lane</i> 3, purified K576D; <i>Lane</i> 3, purified E175N/K576D.</p>