15-HETE-induced ROS promote PASMC cell cycle progression and α-tubulin polymerization in the nucleus.
Qian Li
Min Mao
Yanli Qiu
Gaofeng Liu
Tingting Sheng
Xiufeng Yu
Shuang Wang
Daling Zhu
10.1371/journal.pone.0149164.g005
https://plos.figshare.com/articles/figure/15_HETE_induced_ROS_promote_PASMC_cell_cycle_progression_and_tubulin_polymerization_in_the_nucleus_/2343493
<p>After employing NAC to scavenge ROS in PASMCs, the effects of hypoxia and exogenous 15-HETE on <b>(A)</b> the percentages of cells in the S and G2/M phases were analyzed by flow cytometry (n = 3). <b>(B)</b> α-tubulin polymerization in the nucleus was detected by immunocytochemistry. Scale bars indicate 100 μm. Data are presented as the means ± SEM; *<i>p</i><0.05, **<i>p</i><0.01 versus normal; <b>#</b> <i>p</i><0.05, ##<i>p</i><0.01 versus Hyp+15 (n = 3). Nor, normoxia; Hyp, hypoxia; 15, 15-HETE.</p>
2016-02-12 07:56:29
ROS production
Key Role
hypoxic rats
PASMC
PA
PH
HETE
artery hypertension patients
EC
PVR
reactive oxygen species
Hypoxia Pulmonary Hypertension
cell cycle analyses
incorporation assays
LO
tube formation assays
HPH
muscle cell
p 38 MAPK pathway
mitochondrial electron transport chain