15-HETE-induced ROS promote PASMC cell cycle progression and α-tubulin polymerization in the nucleus. Qian Li Min Mao Yanli Qiu Gaofeng Liu Tingting Sheng Xiufeng Yu Shuang Wang Daling Zhu 10.1371/journal.pone.0149164.g005 https://plos.figshare.com/articles/figure/15_HETE_induced_ROS_promote_PASMC_cell_cycle_progression_and_tubulin_polymerization_in_the_nucleus_/2343493 <p>After employing NAC to scavenge ROS in PASMCs, the effects of hypoxia and exogenous 15-HETE on <b>(A)</b> the percentages of cells in the S and G2/M phases were analyzed by flow cytometry (n = 3). <b>(B)</b> α-tubulin polymerization in the nucleus was detected by immunocytochemistry. Scale bars indicate 100 μm. Data are presented as the means ± SEM; *<i>p</i><0.05, **<i>p</i><0.01 versus normal; <b>#</b> <i>p</i><0.05, ##<i>p</i><0.01 versus Hyp+15 (n = 3). Nor, normoxia; Hyp, hypoxia; 15, 15-HETE.</p> 2016-02-12 07:56:29 ROS production Key Role hypoxic rats PASMC PA PH HETE artery hypertension patients EC PVR reactive oxygen species Hypoxia Pulmonary Hypertension cell cycle analyses incorporation assays LO tube formation assays HPH muscle cell p 38 MAPK pathway mitochondrial electron transport chain