Fibrillation kinetics of insulin incubated in the presence of degradation products of aloin.
Eva S. Lobbens
Vito Foderà
Nils T. Nyberg
Kirsten Andersen
Anna K. Jäger
Lene Jorgensen
Marco van de Weert
10.1371/journal.pone.0149148.g005
https://plos.figshare.com/articles/figure/Fibrillation_kinetics_of_insulin_incubated_in_the_presence_of_degradation_products_of_aloin_/2343472
<p>Representative, normalized fibrillation curves of insulin in the presence of (A) 2% DMSO (green), 400 μM aloin in DMSO (black) and 400 μM aloin heated 10 minutes in DMSO (blue). (B) 0.4% EtOH (red), aloin incubated 1 week in PBS buffer (blue), (C) 2% DMSO (green), 400 μM aloe-emodine (blue) or 400 μM D-glucose (black), (D) 2% DMSO (green), 2% CH<sub>2</sub>Cl<sub>2</sub> (purple), the CH<sub>2</sub>Cl<sub>2</sub> phase of a 3 weeks old 400 μM aloin solution incubated with CH<sub>2</sub>Cl<sub>2</sub> (blue) and the dried CH<sub>2</sub>Cl<sub>2</sub> phase reconstituted in DMSO (black). Fibrillation conditions: ThT assay, pH 7.4.</p>
2016-02-16 07:56:30
compound
Protein Fibrillation May
degradation products
metastable degradation product
insulin fibrillation
transmission electron microscopy
Thioflavin T assays
aloin
Insulin Protein fibrillation