Fibrillation kinetics of insulin incubated in the presence of degradation products of aloin. Eva S. Lobbens Vito Foderà Nils T. Nyberg Kirsten Andersen Anna K. Jäger Lene Jorgensen Marco van de Weert 10.1371/journal.pone.0149148.g005 https://plos.figshare.com/articles/figure/Fibrillation_kinetics_of_insulin_incubated_in_the_presence_of_degradation_products_of_aloin_/2343472 <p>Representative, normalized fibrillation curves of insulin in the presence of (A) 2% DMSO (green), 400 μM aloin in DMSO (black) and 400 μM aloin heated 10 minutes in DMSO (blue). (B) 0.4% EtOH (red), aloin incubated 1 week in PBS buffer (blue), (C) 2% DMSO (green), 400 μM aloe-emodine (blue) or 400 μM D-glucose (black), (D) 2% DMSO (green), 2% CH<sub>2</sub>Cl<sub>2</sub> (purple), the CH<sub>2</sub>Cl<sub>2</sub> phase of a 3 weeks old 400 μM aloin solution incubated with CH<sub>2</sub>Cl<sub>2</sub> (blue) and the dried CH<sub>2</sub>Cl<sub>2</sub> phase reconstituted in DMSO (black). Fibrillation conditions: ThT assay, pH 7.4.</p> 2016-02-16 07:56:30 compound Protein Fibrillation May degradation products metastable degradation product insulin fibrillation transmission electron microscopy Thioflavin T assays aloin Insulin Protein fibrillation