10.1371/journal.pone.0149148.g005 Eva S. Lobbens Eva S. Lobbens Vito Foderà Vito Foderà Nils T. Nyberg Nils T. Nyberg Kirsten Andersen Kirsten Andersen Anna K. Jäger Anna K. Jäger Lene Jorgensen Lene Jorgensen Marco van de Weert Marco van de Weert Fibrillation kinetics of insulin incubated in the presence of degradation products of aloin. Public Library of Science 2016 compound Protein Fibrillation May degradation products metastable degradation product insulin fibrillation transmission electron microscopy Thioflavin T assays aloin Insulin Protein fibrillation 2016-02-16 07:56:30 Figure https://plos.figshare.com/articles/figure/Fibrillation_kinetics_of_insulin_incubated_in_the_presence_of_degradation_products_of_aloin_/2343472 <p>Representative, normalized fibrillation curves of insulin in the presence of (A) 2% DMSO (green), 400 μM aloin in DMSO (black) and 400 μM aloin heated 10 minutes in DMSO (blue). (B) 0.4% EtOH (red), aloin incubated 1 week in PBS buffer (blue), (C) 2% DMSO (green), 400 μM aloe-emodine (blue) or 400 μM D-glucose (black), (D) 2% DMSO (green), 2% CH<sub>2</sub>Cl<sub>2</sub> (purple), the CH<sub>2</sub>Cl<sub>2</sub> phase of a 3 weeks old 400 μM aloin solution incubated with CH<sub>2</sub>Cl<sub>2</sub> (blue) and the dried CH<sub>2</sub>Cl<sub>2</sub> phase reconstituted in DMSO (black). Fibrillation conditions: ThT assay, pH 7.4.</p>