10.1371/journal.pone.0149148.g005
Eva S. Lobbens
Eva
S. Lobbens
Vito Foderà
Vito
Foderà
Nils T. Nyberg
Nils T.
Nyberg
Kirsten Andersen
Kirsten
Andersen
Anna K. Jäger
Anna
K. Jäger
Lene Jorgensen
Lene
Jorgensen
Marco van de Weert
Marco
van de Weert
Fibrillation kinetics of insulin incubated in the presence of degradation products of aloin.
Public Library of Science
2016
compound
Protein Fibrillation May
degradation products
metastable degradation product
insulin fibrillation
transmission electron microscopy
Thioflavin T assays
aloin
Insulin Protein fibrillation
2016-02-16 07:56:30
Figure
https://plos.figshare.com/articles/figure/Fibrillation_kinetics_of_insulin_incubated_in_the_presence_of_degradation_products_of_aloin_/2343472
<p>Representative, normalized fibrillation curves of insulin in the presence of (A) 2% DMSO (green), 400 μM aloin in DMSO (black) and 400 μM aloin heated 10 minutes in DMSO (blue). (B) 0.4% EtOH (red), aloin incubated 1 week in PBS buffer (blue), (C) 2% DMSO (green), 400 μM aloe-emodine (blue) or 400 μM D-glucose (black), (D) 2% DMSO (green), 2% CH<sub>2</sub>Cl<sub>2</sub> (purple), the CH<sub>2</sub>Cl<sub>2</sub> phase of a 3 weeks old 400 μM aloin solution incubated with CH<sub>2</sub>Cl<sub>2</sub> (blue) and the dried CH<sub>2</sub>Cl<sub>2</sub> phase reconstituted in DMSO (black). Fibrillation conditions: ThT assay, pH 7.4.</p>