10.1371/journal.pone.0047472.g009 Sandeep S. Patil Sandeep S. Patil Ivaylo Gentschev Ivaylo Gentschev Marion Adelfinger Marion Adelfinger Ulrike Donat Ulrike Donat Michael Hess Michael Hess Stephanie Weibel Stephanie Weibel Ingo Nolte Ingo Nolte Alexa Frentzen Alexa Frentzen Aladar A. Szalay Aladar A. Szalay Presence of immune cells in tumors of STSA-1 xenografted mice 7 days after GLV-1h68-, GLV-1h109- or PBS-treatments. Public Library of Science 2012 cells tumors stsa-1 xenografted mice days glv-1h109- 2012-10-16 02:32:58 Figure https://plos.figshare.com/articles/figure/_Presence_of_immune_cells_in_tumors_of_STSA_1_xenografted_mice_7_days_after_GLV_1h68_GLV_1h109_or_PBS_treatments_/229178 <p>Percentage of (A) Gr-1<sup>high</sup>CD11b<sup>+</sup> (MDSCs, granulocytes), (B) Gr-1<sup>int</sup>CD11b<sup>+</sup> (MDSCs, monocytes), (C) F4/80<sup>+</sup>CD45<sup>+</sup> (macrophages) or (D) MHCII<sup>+</sup>CD45<sup>+</sup> (mainly B cells, macrophages and dendritic cells) cells in tumors of STSA-1 xenografted mice. Experiments were done twice with at least 3 mice per group. The data are presented as mean values +/- SD. The statistical significance was analyzed using one-way ANOVA followed Bonferronìs multiple comparison test (***P<0.001, **P<0.01, *P<0.05). The Anti-Gr-1 mAb (RB6-8C5) has long been used to stain MDSCs and allows the distinction of at least two subsets of granulocytes (Gr-1<sup>high</sup>CD11b<sup>+</sup>) and monocytic cells (Gr-1<sup>int</sup>CD11b<sup>+</sup>).</p>