%0 Figure %A S. Hopp, Christine %A Flueck, Christian %A Solyakov, Lev %A Tobin, Andrew %A A. Baker, David %D 2012 %T Parasite morphology and global protein phosphorylation pattern of PKG inhibitor-treated P. falciparum schizonts. %U https://plos.figshare.com/articles/figure/_Parasite_morphology_and_global_protein_phosphorylation_pattern_of_PKG_inhibitor_treated_P_falciparum_schizonts_/218996 %R 10.1371/journal.pone.0048206.g003 %2 https://plos.figshare.com/ndownloader/files/548506 %K morphology %K phosphorylation %K pkg %K inhibitor-treated %X

(A) Immunofluorescent staining of DMSO/compound 1-treated WT schizonts using antibodies detecting (i) PfGAP45 [34], (ii) PfSUB1 [14] and (iii) PfAMA1 [23]. Representative images are shown for each staining together with parasite nuclei stained with DAPI. Bars ∼5 µM. (B) Metabolic labelling of phosphoproteins in P. falciparum schizonts. Autoradiographs of (i) 3D7 WT and (ii) gatekeeper mutant 3D7 PfPKGT618Q schizonts, treated with 32P-orthophosphate and DMSO (−) or compound 2 (+) prior to lysis, ÄKTA anion exchange chromatography (fractions 10–14 are shown) and separation by SDS-PAGE. Rectangular boxes highlight bands that show a differential signal following inhibitor-treatment in WT, but not PfPKGT618Q schizonts.

%I PLOS ONE