S. Vinay, Dass Lee, Seung J. Kim, Chang H. Sik Oh, Ho S. Kwon, Byoung Activation upregulates 4-1BB expression in PDCA-1<sup>+</sup> cells. <p><b>A</b> Total BM-derived cells were stimulated with the indicated soluble agonists for 3d, stained with fluorochrome- tagged Abs to 4-1BB, PDCA-1, and IgD, then analysed by flow cytometry. The percentages of 4-1BB<sup>+</sup> cells in gated PDCA-1<sup>+</sup>IgD<sup>+</sup> and PDCA-1<sup>+</sup>IgD<sup>−</sup> fractions are presented as bar graphs (mean ± SD). The results of three pooled independent experiments are shown (<i>n</i> = 3). <b>B</b> Purified splenic PDCA-1<sup>+</sup> B cells from naïve B6 mice were stimulated with anti-4-1BB (5 µg/ml) for 3d, stained as indicated, and flow cytometry was performed. The frequencies of cells expressing the indicated activation markers were evaluated and are presented as bar graphs (mean ± SD). The results of three pooled independent experiments are shown (<i>n</i> = 3). * <i>p</i><0.05.</p> upregulates;4-1bb 2013-02-19
    https://plos.figshare.com/articles/figure/_Activation_upregulates_4_1BB_expression_in_PDCA_1_cells_/208996
10.1371/journal.pone.0050272.g002