Oenothein B directly primes purified human NK cells to produce IFNγ. Andrew G. Ramstead Igor A. Schepetkin Mark T. Quinn Mark A. Jutila 10.1371/journal.pone.0050546.g008 https://plos.figshare.com/articles/figure/_Oenothein_B_directly_primes_purified_human_NK_cells_to_produce_IFN_947_/203337 <p>Human NK cells (5×10<sup>4</sup> cells/well) were sorted and treated with 20 µg/ml oenothein B, 100 ng/ml rhu IL-18, both, or medium alone. After 24 hrs, soluble IFNγ was measured by ELISA. The graph represents soluble IFNγ levels in culture supernatant fluids from three separate experiments with three different donors. Error bars indicate SEM. Each sample was analyzed in triplicate. Significance was determined by One-way ANOVA with Bonferroni post-test. *p<0.05, **p<0.01, ***p<0.001.</p> 2012-11-30 00:55:37 primes purified nk cells