Allopurinol and uricase pre-treatment attenuate alveolar barrier dysfunction. Maria T. Kuipers Hamid Aslami Alexander P. J. Vlaar Nicole P. Juffermans Anita M. Tuip-de Boer Maria A. Hegeman Geartsje Jongsma Joris J. T. H. Roelofs Tom van der Poll Marcus J. Schultz Catharina W. Wieland 10.1371/journal.pone.0050559.g007 https://plos.figshare.com/articles/figure/_Allopurinol_and_uricase_pre_treatment_attenuate_alveolar_barrier_dysfunction_/203331 <p>Mice were pre-treated with vehicle (10% dimethylsulfoxide, dark grey bars), uricase (0.2 mg/kg, light grey, striped bars), or allopurinol (25 mg/kg (white bars) 1 hour before start of mechanical ventilation (VILI). Spontaneously breathing, vehicle pre-treated mice served as controls (C). After 5 hours mice were killed. Lung wet-to-dry ratio (<b>A</b>), total protein levels in lung lavage fluid (<b>B</b>) and immunoglobulin M (IgM) concentrations in (<b>C</b>) were analyzed. Data represent mean ± SEM of 4 control mice and n = 9 for the VILI groups (for IgM n = 5−6). ##P<0.01, ###P<0.001 vs. vehicle ventilated. ***p<0.001 vs. vehicle control.</p> 2012-11-30 00:55:31 uricase pre-treatment attenuate alveolar