10.1371/journal.pone.0050809.g002
Oliver Jungmann
Oliver
Jungmann
Katerina Nikolovska
Katerina
Nikolovska
Christian Stock
Christian
Stock
Jan-Niklas Schulz
Jan-Niklas
Schulz
Beate Eckes
Beate
Eckes
Christoph Riethmüller
Christoph
Riethmüller
Rick T. Owens
Rick
T. Owens
Renato V. Iozzo
Renato
V. Iozzo
Daniela G. Seidler
Daniela
G. Seidler
Expression of vimentin in 3D cultures of Dcn<sup>−/−</sup> fibroblasts treated with either decorin or decorin core (core) at day 6.
Public Library of Science
2012
vimentin
3d
cultures
fibroblasts
treated
decorin
2012-12-03 00:33:46
Figure
https://plos.figshare.com/articles/figure/_Expression_of_vimentin_in_3D_cultures_of_Dcn_8722_8722_fibroblasts_treated_with_either_decorin_or_decorin_core_core_at_day_6_/202026
<p>Immunofluorescence staining of vimentin (A), decorin (C) and collagen type I (D) in µ-slide VI, visualized by Alexa 488 conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). Images show merged z-axis layers of the complete 3D matrix. B: Quantification of vimentin immunofluorescence signal in merged layers normalized to cell number (nuclei) per image. Data represent 5 independent experiments and are expressed as mean ± SD (for each condition and experiments, 15 images were evaluated; **, p<0.01). Bar = 100 µm.</p>