10.1371/journal.pone.0050809.g002 Oliver Jungmann Oliver Jungmann Katerina Nikolovska Katerina Nikolovska Christian Stock Christian Stock Jan-Niklas Schulz Jan-Niklas Schulz Beate Eckes Beate Eckes Christoph Riethmüller Christoph Riethmüller Rick T. Owens Rick T. Owens Renato V. Iozzo Renato V. Iozzo Daniela G. Seidler Daniela G. Seidler Expression of vimentin in 3D cultures of Dcn<sup>−/−</sup> fibroblasts treated with either decorin or decorin core (core) at day 6. Public Library of Science 2012 vimentin 3d cultures fibroblasts treated decorin 2012-12-03 00:33:46 Figure https://plos.figshare.com/articles/figure/_Expression_of_vimentin_in_3D_cultures_of_Dcn_8722_8722_fibroblasts_treated_with_either_decorin_or_decorin_core_core_at_day_6_/202026 <p>Immunofluorescence staining of vimentin (A), decorin (C) and collagen type I (D) in µ-slide VI, visualized by Alexa 488 conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). Images show merged z-axis layers of the complete 3D matrix. B: Quantification of vimentin immunofluorescence signal in merged layers normalized to cell number (nuclei) per image. Data represent 5 independent experiments and are expressed as mean ± SD (for each condition and experiments, 15 images were evaluated; **, p<0.01). Bar = 100 µm.</p>