1,25(OH)2D3 administration regulated the protein abundance of NaPi-IIa, klotho and Vdr.

Representative western blots of total renal protein homogenates from mice injected with 1,25(OH)2D3 either once within 14 hours (14h) or twice within 96 hours (96h) and the corresponding controls using antibodies against NaPi-IIa (A), full length Klotho (B), Cyp24a1 (C) and Vdr (D). The proteins of interest are shown in the top panels, whereas the corresponding β-actin signal is shown in the bottom image. Densitometric quantification was normalized to the corresponding β-actin values. Bar graphs represent mean ± SD (n = 6/group) of the normalized values and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05, ** p ≤ 0.01 and *** p < 0.0001.