1,25(OH)₂D₃ administration regulated the protein abundance of NaPi-IIa, klotho and Vdr.

Representative western blots of total renal protein homogenates from mice injected with 1,25(OH)₂D₃ either once within 14 hours (14h) or twice within 96 hours (96h) and the corresponding controls using antibodies against NaPi-IIa (A), full length Klotho (B), Cyp24a1 (C) and Vdr (D). The proteins of interest are shown in the top panels, whereas the corresponding β-actin signal is shown in the bottom image. Densitometric quantification was normalized to the corresponding β-actin values. Bar graphs represent mean ± SD (n = 6/group) of the normalized values and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05, ** p ≤ 0.01 and *** p < 0.0001.