10.1371/journal.pone.0051611.g004 Melissa M. Singh Melissa M. Singh Adrienne Howard Adrienne Howard Mary E. Irwin Mary E. Irwin Yin Gao Yin Gao Xiaolin Lu Xiaolin Lu Asha Multani Asha Multani Joya Chandra Joya Chandra Fyn contributes to the growth and clonogenicity of BCR-ABL1 expressing bone marrow cells. Public Library of Science 2013 contributes clonogenicity bcr-abl1 expressing marrow 2013-02-19 18:33:37 Figure https://plos.figshare.com/articles/figure/_Fyn_contributes_to_the_growth_and_clonogenicity_of_BCR_ABL1_expressing_bone_marrow_cells_/194211 <p>(A) Bone marrow cells from wild-type (Fyn<sup>+/+</sup>) or Fyn knockout (Fyn<sup>−/−</sup>) mice were transduced with vector (GFP) or vector expressing p210 BCR-ABL1 (p210). Expression of p210 and Fyn was detected in total protein lysates (100 µg) using antibodies specific for Abl and Fyn, respectively. Actin was used as a loading control. The blot is representative of three independent experiments. (B) Transduced bone marrow cells were plated in methylcellulose soft agar and colonies were counted after five days. *p<0.01, n = 3, mean+SEM. (C) Transduced bone marrow cells were plated in 96-well plates at 150,000 cells/mL. Every two days, cells were stained with trypan blue and viable cells were counted to determine growth rate. *p<0.001, n = 5, mean+SEM.</p>