10.1371/journal.pone.0051611.g004
Melissa M. Singh
Melissa
M. Singh
Adrienne Howard
Adrienne
Howard
Mary E. Irwin
Mary
E. Irwin
Yin Gao
Yin
Gao
Xiaolin Lu
Xiaolin
Lu
Asha Multani
Asha
Multani
Joya Chandra
Joya
Chandra
Fyn contributes to the growth and clonogenicity of BCR-ABL1 expressing bone marrow cells.
Public Library of Science
2013
contributes
clonogenicity
bcr-abl1
expressing
marrow
2013-02-19 18:33:37
Figure
https://plos.figshare.com/articles/figure/_Fyn_contributes_to_the_growth_and_clonogenicity_of_BCR_ABL1_expressing_bone_marrow_cells_/194211
<p>(A) Bone marrow cells from wild-type (Fyn<sup>+/+</sup>) or Fyn knockout (Fyn<sup>−/−</sup>) mice were transduced with vector (GFP) or vector expressing p210 BCR-ABL1 (p210). Expression of p210 and Fyn was detected in total protein lysates (100 µg) using antibodies specific for Abl and Fyn, respectively. Actin was used as a loading control. The blot is representative of three independent experiments. (B) Transduced bone marrow cells were plated in methylcellulose soft agar and colonies were counted after five days. *p<0.01, n = 3, mean+SEM. (C) Transduced bone marrow cells were plated in 96-well plates at 150,000 cells/mL. Every two days, cells were stained with trypan blue and viable cells were counted to determine growth rate. *p<0.001, n = 5, mean+SEM.</p>