10.1371/journal.pone.0052764.g004
Juliane C. Campos
Juliane
C. Campos
Bruno B. Queliconi
Bruno
B. Queliconi
Paulo M. M. Dourado
Paulo
M. M. Dourado
Telma F. Cunha
Telma
F. Cunha
Vanessa O. Zambelli
Vanessa
O. Zambelli
Luiz R. G. Bechara
Luiz
R. G. Bechara
Alicia J. Kowaltowski
Alicia
J. Kowaltowski
Patricia C. Brum
Patricia
C. Brum
Daria Mochly-Rosen
Daria
Mochly-Rosen
Julio C. B. Ferreira
Julio
C. B. Ferreira
4-HNE irreversibly inactivates 20S proteasome <i>in vitro</i>.
Public Library of Science
2013
irreversibly
inactivates
20s
proteasome
2013-02-19 17:59:44
Figure
https://plos.figshare.com/articles/figure/_4_HNE_irreversibly_inactivates_20S_proteasome_in_vitro_/188198
<p>(A) Schematic panel of <i>in vitro</i> incubations. (B) Purified 20S proteasome (1 ug) was incubated for 30 min at 37°C with 4-HNE (10 or 100 µM) and proteasomal activity was measured at the end of incubation. DTT (1μ) was added to the reaction either previous or after 4-HNE incubations. Of interest, prior, but no later, incubation with DTT protected 4-hydroxi-2-nonenal inhibition of proteasomal activity. Error bars indicate SEM. Proteasomal activity [F (7, 32) = 21.37, p<0.0001]. *, p<0.05 vs. control, 4-HNE (10 µM)+DTT (before). #, p<0.05 vs. 4-HNE (10 µM).</p>