10.1371/journal.pone.0052764.g004 Juliane C. Campos Juliane C. Campos Bruno B. Queliconi Bruno B. Queliconi Paulo M. M. Dourado Paulo M. M. Dourado Telma F. Cunha Telma F. Cunha Vanessa O. Zambelli Vanessa O. Zambelli Luiz R. G. Bechara Luiz R. G. Bechara Alicia J. Kowaltowski Alicia J. Kowaltowski Patricia C. Brum Patricia C. Brum Daria Mochly-Rosen Daria Mochly-Rosen Julio C. B. Ferreira Julio C. B. Ferreira 4-HNE irreversibly inactivates 20S proteasome <i>in vitro</i>. Public Library of Science 2013 irreversibly inactivates 20s proteasome 2013-02-19 17:59:44 Figure https://plos.figshare.com/articles/figure/_4_HNE_irreversibly_inactivates_20S_proteasome_in_vitro_/188198 <p>(A) Schematic panel of <i>in vitro</i> incubations. (B) Purified 20S proteasome (1 ug) was incubated for 30 min at 37°C with 4-HNE (10 or 100 µM) and proteasomal activity was measured at the end of incubation. DTT (1μ) was added to the reaction either previous or after 4-HNE incubations. Of interest, prior, but no later, incubation with DTT protected 4-hydroxi-2-nonenal inhibition of proteasomal activity. Error bars indicate SEM. Proteasomal activity [F (7, 32) = 21.37, p<0.0001]. *, p<0.05 vs. control, 4-HNE (10 µM)+DTT (before). #, p<0.05 vs. 4-HNE (10 µM).</p>