Xu, Qilong Shi, Yanlin Dadhwal, Poonam Ting Liang, Fang <i>trans-</i>Complementation of the <i>rpoS</i> mutant and modification of the mutant to constitutively express OspC and to simultaneously express OspC, DbpA and DbpB. <p>(A&B) Construction of pBBE22-<i>rpoS</i> and pME22-<i>C</i>′<i>B</i>′<i>A</i>′. All restriction enzyme sites and primer binding sites used for plasmid construction are labeled. (C) Confirmation of OspC, DbpA and DbpB production by immunoblotting. The parental clone 13A and transformants Δ<i>rpoS/E22</i>, Δ<i>rpoS/rpoS</i>, Δ<i>rpoS/ospC′</i>, Δ<i>rpoS/C′B′A′</i> and 13A<i>/C′B′A′</i> were grown to late-log phase in BSK-H complete medium, harvested by centrifugation and subjected to immunoblot analyses probed with a mixture of FlaB mAb and OspC mAb (top), or mouse anti-DbpA (middle) or -DbpB sera (bottom).</p> mutant;modification;constitutively;ospc;dbpa 2013-02-19
    https://plos.figshare.com/articles/figure/_trans_Complementation_of_the_rpoS_mutant_and_modification_of_the_mutant_to_constitutively_express_OspC_and_to_simultaneously_express_OspC_DbpA_and_DbpB_/187784
10.1371/journal.pone.0053212.g002