Smadja Storz, Sima Tovin, Adi Mracek, Philipp Alon, Shahar S. Foulkes, Nicholas Gothilf, Yoav CK1δ inhibition disrupts the PAC-2 cell circadian clock. <p>Dose-response of PF-670462, a pan-CK1δ/ε inhibitor and PF-4800567, a selective inhibitor of CK1ε, in the zebrafish PAC-2 cell line. Bioluminescence assay of cells transfected with per1b:Luc (A and C) or Ebox:Luc (B and D), and treated with PF-670462 (A and B) or with PF-4800567 (C and D) at the indicated time (arrow). Cells were maintained under LD cycles, and then the inhibitor was added to the cell culture 1.5 h before lights on (black arrows) at different concentrations (PF-670462: 0.5 μM- light blue line, 1 μM- blue line, 2 μM-green line, 5 μM- red line. PF-4800567: 5 μM- green line, 7.5 μM- light blue line and 10 μM- blue line). Controls were treated with DMSO (black line) or with culture medium (gray line). After three LD cycles cell were transferred to DD. Bioluminescence is plotted on the y-axis and time (hours) on the x-axis. White/black bars show the light and dark periods, respectively. Clock-controlled rhythmic promoter was disrupted by PF-67046 but not by PF-4800567, thus CK1δ activity appears to be important for peripheral circadian clock function.</p> CK 1δ activity;PAC;CK 1δ inhibition;circadian clock system;aanat 2 mRNA expression;locomotor activity;AANAT;PF;Zebrafish Circadian Timing System Zebrafish;circadian timing system;PER;pineal gland;circadian timing mechanism;CK 1ε inhibitor;CK 1δ;CK 1ε 2013-01-21
    https://plos.figshare.com/articles/dataset/_CK1_948_inhibition_disrupts_the_PAC_2_cell_circadian_clock_/175063
10.1371/journal.pone.0054189.g001