Effects of buffer ionic composition on the native structure of PaBADH and SoBADH. Georgina Garza-Ramos Carlos Mújica-Jiménez Rosario A. Muñoz-Clares 10.1371/journal.pone.0054899.g002 https://plos.figshare.com/articles/figure/_Effects_of_buffer_ionic_composition_on_the_native_structure_of_PaBADH_and_SoBADH_/172598 <p>(<b>A</b> and <b>B</b>) SEC elution profiles of PaBADH (solid line) and SoBADH (dotted line) in 1 mM Pipes-TEAOH, pH 6.9, containing 1 mM EDTA and 0.5 mM DTT (non-salt buffer) (B), or in this buffer plus 250 mM KCl (K-buffer) (<b>A</b>). Arrows denote the positions of molecular-mass standards, from <i>left</i> to <i>right</i>: bovine tyroglobulin (670 kDa), bovine liver catalase (240 kDa), bovine gamma-globulin (158 kDa) and horse myoglobin (17 kDa). (<b>C</b> and <b>D</b>) DLS measurements. Hydrodynamic radius distribution plots of PaBADH (<i>top</i> of panels C and D) and SoBADH (<i>bottom</i> of panels C and D) in K-buffer (<b>C</b>) and non-salt buffer (D). Histograms are representative of volume-weighted size distributions of 0.25 mg/ml enzyme samples.</p> 2013-01-24 00:43:18 buffer ionic pabadh