Activation of p53 and <i>Trp53</i>-dependent proliferation arrest in <i>Zfp148<sup>gt/gt</sup></i> MEFs. Volkan I. Sayin Anna Nilton Mohamed X. Ibrahim Pia Ågren Erik Larsson Marleen M. Petit Lillemor Mattsson Hultén Marcus Ståhlman Bengt R. Johansson Martin O. Bergo Per Lindahl 10.1371/journal.pone.0055720.g006 https://plos.figshare.com/articles/figure/_Activation_of_p53_and_Trp53_dependent_proliferation_arrest_in_Zfp148_gt_gt_MEFs_/164888 <p>(<b>A</b>) Western blots of MEF lysates showing expression of phospho-p53<sup>Ser18</sup> in wt and <i>Zfp148<sup>gt/gt</sup></i> MEFs cultured at 21 or 3% oxygen, respectively. <i>Trp53<sup>−/−</sup></i> cells were used as a negative control and actin was used as a loading control. Asterisk indicates unspecific band. (<b>B</b>) Real-time RT-PCR of <i>p21</i> in wt and <i>Zfp148<sup>gt/gt</sup></i> MEFs cultured at 21 or 3% oxygen, respectively, and on <i>Trp53<sup>+/+</sup></i>, <i>Trp53<sup>+/−</sup></i> and <i>Trp53<sup>−/−</sup></i> genetic backgrounds (<i>n</i> = 4). (<b>C, D</b>) CPD of <i>Zfp148<sup>gt/gt</sup></i> and wt MEFs on <i>Trp53<sup>+/+</sup></i> or <i>Trp53<sup>−/−</sup></i> (C) and <i>Trp53<sup>+/+</sup></i> or <i>Trp53<sup>+/−</sup></i> (D) genetic backgrounds (<i>n</i> = 3). (<b>E, F</b>) CPD of <i>Zfp148<sup>gt/gt</sup></i> and wt MEFs supplemented with <i>n</i>-acetyl-L-cysteine (NAC) (E) in the culture medium (<i>n</i> = 4), or cultured at atmospheric (21%) or low (3%) oxygen concentrations (F) (<i>n</i> = 3). *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001.</p> 2013-02-06 01:21:28 p53 proliferation