10.1371/journal.pone.0055720.g006
Volkan I. Sayin
Volkan
I. Sayin
Anna Nilton
Anna
Nilton
Mohamed X. Ibrahim
Mohamed
X. Ibrahim
Pia Ågren
Pia
Ågren
Erik Larsson
Erik
Larsson
Marleen M. Petit
Marleen
M. Petit
Lillemor Mattsson Hultén
Lillemor
Mattsson Hultén
Marcus Ståhlman
Marcus
Ståhlman
Bengt R. Johansson
Bengt
R. Johansson
Martin O. Bergo
Martin
O. Bergo
Per Lindahl
Per
Lindahl
Activation of p53 and <i>Trp53</i>-dependent proliferation arrest in <i>Zfp148<sup>gt/gt</sup></i> MEFs.
Public Library of Science
2013
p53
proliferation
2013-02-06 01:21:28
Figure
https://plos.figshare.com/articles/figure/_Activation_of_p53_and_Trp53_dependent_proliferation_arrest_in_Zfp148_gt_gt_MEFs_/164888
<p>(<b>A</b>) Western blots of MEF lysates showing expression of phospho-p53<sup>Ser18</sup> in wt and <i>Zfp148<sup>gt/gt</sup></i> MEFs cultured at 21 or 3% oxygen, respectively. <i>Trp53<sup>−/−</sup></i> cells were used as a negative control and actin was used as a loading control. Asterisk indicates unspecific band. (<b>B</b>) Real-time RT-PCR of <i>p21</i> in wt and <i>Zfp148<sup>gt/gt</sup></i> MEFs cultured at 21 or 3% oxygen, respectively, and on <i>Trp53<sup>+/+</sup></i>, <i>Trp53<sup>+/−</sup></i> and <i>Trp53<sup>−/−</sup></i> genetic backgrounds (<i>n</i> = 4). (<b>C, D</b>) CPD of <i>Zfp148<sup>gt/gt</sup></i> and wt MEFs on <i>Trp53<sup>+/+</sup></i> or <i>Trp53<sup>−/−</sup></i> (C) and <i>Trp53<sup>+/+</sup></i> or <i>Trp53<sup>+/−</sup></i> (D) genetic backgrounds (<i>n</i> = 3). (<b>E, F</b>) CPD of <i>Zfp148<sup>gt/gt</sup></i> and wt MEFs supplemented with <i>n</i>-acetyl-L-cysteine (NAC) (E) in the culture medium (<i>n</i> = 4), or cultured at atmospheric (21%) or low (3%) oxygen concentrations (F) (<i>n</i> = 3). *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001.</p>