10.1371/journal.pone.0055720.g006 Volkan I. Sayin Volkan I. Sayin Anna Nilton Anna Nilton Mohamed X. Ibrahim Mohamed X. Ibrahim Pia Ågren Pia Ågren Erik Larsson Erik Larsson Marleen M. Petit Marleen M. Petit Lillemor Mattsson Hultén Lillemor Mattsson Hultén Marcus Ståhlman Marcus Ståhlman Bengt R. Johansson Bengt R. Johansson Martin O. Bergo Martin O. Bergo Per Lindahl Per Lindahl Activation of p53 and <i>Trp53</i>-dependent proliferation arrest in <i>Zfp148<sup>gt/gt</sup></i> MEFs. Public Library of Science 2013 p53 proliferation 2013-02-06 01:21:28 Figure https://plos.figshare.com/articles/figure/_Activation_of_p53_and_Trp53_dependent_proliferation_arrest_in_Zfp148_gt_gt_MEFs_/164888 <p>(<b>A</b>) Western blots of MEF lysates showing expression of phospho-p53<sup>Ser18</sup> in wt and <i>Zfp148<sup>gt/gt</sup></i> MEFs cultured at 21 or 3% oxygen, respectively. <i>Trp53<sup>−/−</sup></i> cells were used as a negative control and actin was used as a loading control. Asterisk indicates unspecific band. (<b>B</b>) Real-time RT-PCR of <i>p21</i> in wt and <i>Zfp148<sup>gt/gt</sup></i> MEFs cultured at 21 or 3% oxygen, respectively, and on <i>Trp53<sup>+/+</sup></i>, <i>Trp53<sup>+/−</sup></i> and <i>Trp53<sup>−/−</sup></i> genetic backgrounds (<i>n</i> = 4). (<b>C, D</b>) CPD of <i>Zfp148<sup>gt/gt</sup></i> and wt MEFs on <i>Trp53<sup>+/+</sup></i> or <i>Trp53<sup>−/−</sup></i> (C) and <i>Trp53<sup>+/+</sup></i> or <i>Trp53<sup>+/−</sup></i> (D) genetic backgrounds (<i>n</i> = 3). (<b>E, F</b>) CPD of <i>Zfp148<sup>gt/gt</sup></i> and wt MEFs supplemented with <i>n</i>-acetyl-L-cysteine (NAC) (E) in the culture medium (<i>n</i> = 4), or cultured at atmospheric (21%) or low (3%) oxygen concentrations (F) (<i>n</i> = 3). *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001.</p>